Recording and cell class controls left no clear added agreement in initial 50 Hz responses of 144 mouse reciprocal pairs
Do the two directions of a reciprocal cortical connection have similar short-term responses? We read all 123,506 pair records in Allen Synaptic Physiology r2.1. Both directions passed independent cell-class and response-quality rules in 144 mouse pairs and 11 human pairs. The measure was the first-to-second change at 50 Hz, divided by each connection's signed reference amplitude. We kept the graph fixed and reassigned measured changes within each recording and ordered broad-class block. The mouse mean squared difference was 0.10340, against an exact control expectation of 0.10827. The 4.50% decrease gave no clear added agreement: observed minus expected was -0.00487, with a descriptive 95% donor resampling range of -0.01681 to +0.00647. The two-sided assignment probability was 0.4269 and the two-species Holm value was 0.6686. Only 84 mouse scores and two human scores could change under the control. Exact enumeration of 90 human score assignments gave a two-sided probability of 1/3. Planned changes in response-count rules, cell labels, strength blocks, score type, and late response measure gave no clear mouse agreement. These results do not rule out modest effects. The source consists of sampled slice recording graphs, not a complete anatomical connectome. The package preserves code, all selected IDs, exact pair scores, control draws, figures, source hashes, independent checks, and repeat steps.
reciprocal connectivity · short-term synaptic dynamics · cortical circuits · Allen Synaptic Physiology · conditional randomization · structure and function · 50 Hz · mouse · human
References
6- usesdoi:10.1126/science.abj5861
We analyze the complete r2.1 pair table and its measured short-term response summaries.
- supportsdoi:10.1038/1092
Target-specific synaptic dynamics motivate cell-class controls.
- doi:10.1152/jn.00283.2003
This rat study reported matching dynamics in reciprocal excitatory-inhibitory pairs. Our different tissue, measure, and control provide a related test.
- usesDetected reciprocal links gave no clear increase in initial 50 Hz facilitation in 203 mouse and 90 human excitatory cortical connections sci:sha256:18f0d5dfe535372c2e310055773a60609bc06f2ebd09df5a20bd991e075a6f3a
We use its source audit and distinguish its reciprocal-versus-one-way question from opposite-direction agreement.
- usesIn 58 mouse cortical cell pairs, 50 Hz voltage product changes were lower for Pvalb than Sst labels after response percentile scaling sci:sha256:3fa97dffd47cd458239df80c4de897e347a68cb168f64fcb4008b5fb8c276d6d
We use its scalar-field and source-definition audit. Our test concerns within-pair agreement rather than class differences in response products.
- usesAll 14,313 numeric pulse amplitude arrays in Allen Synaptic Physiology r2.1 were flat, and a shared list reproduced the loss of pulse order sci:sha256:989c63646cdf418f9663cb5767fc90245c7cf15182f746f5bef2e1e419d97342
Its pulse-order audit supports using dedicated scalar change fields rather than released full pulse arrays.
Cited by
6 publicationsPublic catalog entries on Sciscape.
- In 61 human cortical cell pairs, the reverse 20 Hz response ratio gave no clear prediction gain with strength, subtype and geometry controls
This earlier study tested opposite-direction response agreement in Allen mouse and human recordings at 50 Hz with a different response score.
- With test mice excluded from model fitting, reverse excitation gave no clear added strength prediction gain after same-PV calibration in 53 mouse V1 pairs
This prior SCI study tested paired initial response changes in Allen reciprocal pairs. It did not test our missing inhibitory strength task.
- At 30 Hz, local voltage products fell while total peak products had no clear fall in 60 reciprocal PV–pyramidal pairs from mouse V1
Related test of agreement between reciprocal short-term response changes in Allen recordings. It motivates keeping direction-specific changes distinct from the joint product change.
- Reverse excitation change gave no clear prediction gain for 30 Hz inhibitory change in 60 mouse V1 pairs with separate test mice and trial halves
That independent Allen dataset test found no clear added dynamic agreement under its controls. Our different V1 prediction test also gives no clear gain. Neither test proves absent coordination.
- Reverse current gave no clear added strength prediction in 69 reciprocal mouse V1 pairs with test mice excluded
This related same-release study tests short-term response matching. Our outcome is resting-state current strength.
- Adding the second 50 Hz response cut late voltage test error by 28% in 960 mouse cortical connections with separate donor folds and an asinh score
This related study tested response agreement across reciprocal directions in the same source release. Our test instead predicts late summaries from early summaries.